ULB/11 September 2009

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(Difference between revisions)
(New page: Système 1 Repiquage des colonies et analyse au microscope. Préparer les tubes pour le séquençage (40µl) : - hfsG - hfsH - H + Ter - G + H + Ter - Promoteur + RBS + RFP - PRR + GHT - c...)
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Système 1
Système 1
Repiquage des colonies et analyse au microscope.
Repiquage des colonies et analyse au microscope.

Revision as of 08:26, 5 October 2009

iGEM Team:ULB-Brussels Wiki

Système 1 Repiquage des colonies et analyse au microscope. Préparer les tubes pour le séquençage (40µl) : - hfsG - hfsH - H + Ter - G + H + Ter - Promoteur + RBS + RFP - PRR + GHT - ccdA I - ccdA F Y a-t-il oui ou non du lactose dans le milieu de culture LB ?

Système 3 Extraction des plasmides des tubes 4 et 5. Digestion et ligation des briques du Quorum Sensing (système 3A). Trouver un plasmide qu’on a en stock avec une ORI différente et mettre en place le syst ème 3B. Est-il possible de thermoporer 2 plasmides en même temps ? Faisable selon Gilles.

Système 2 Repiquage des ligations 9-10.

Faire les ligations tubes 4 (K14...) avec tube 7 (GFP) afin de tester si c2P22 réprime bien K14...

Pour changer de milieu de culture : centrifuger les bactéries, laver avec du milieu LB, centrifuger à nouveau et ensuite mettre le nouveau milieu de culture.