ULB/11 September 2009
From 2009.igem.org
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+ | '''System 1 ''' | ||
- | + | *Transplanting of colonies and microscopic analysis. | |
- | + | *Prepare tubes for sequencing : | |
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- | + | **HfsG | |
- | + | **HfsH | |
- | + | **HfsH + Ter | |
- | + | **HfsG + HfsH + Ter | |
- | + | **Promoter + RBS + RFP | |
+ | **PRR + GHT | ||
+ | **I ccdA | ||
+ | **CcdA F Y | ||
- | + | '''System 3 ''' | |
- | + | ||
- | + | *Extraction of plasmids tubes 4 and 5. | |
- | + | *Digestion and ligation of Quorum Sensing brick. | |
+ | |||
+ | '''System 2 ''' | ||
+ | |||
+ | *Transplanting ligations 9-10. | ||
+ | |||
+ | *Make ligation tube 4 with tube 7 to test if c2P22 well represses K145150. |
Revision as of 09:55, 7 October 2009
- Transplanting of colonies and microscopic analysis.
- Prepare tubes for sequencing :
- HfsG
- HfsH
- HfsH + Ter
- HfsG + HfsH + Ter
- Promoter + RBS + RFP
- PRR + GHT
- I ccdA
- CcdA F Y
System 3
- Extraction of plasmids tubes 4 and 5.
- Digestion and ligation of Quorum Sensing brick.
System 2
- Transplanting ligations 9-10.
- Make ligation tube 4 with tube 7 to test if c2P22 well represses K145150.