ULB/11 September 2009
From 2009.igem.org
- Transplanting of colonies and microscopic analysis.
- Prepare tubes for sequencing :
- HfsG
- HfsH
- HfsH + Ter
- HfsG + HfsH + Ter
- Promoter + RBS + RFP
- PRR + GHT
- I ccdA
- CcdA F Y
System 3
- Extraction of plasmids tubes 4 and 5.
- Digestion and ligation of Quorum Sensing brick.
System 2
- Transplanting ligations 9-10.
- ligation of tube 4 with tube 7 to test if c2P22 well represses K145150.