ULB/11 September 2009

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iGEM Team:ULB-Brussels Wiki

System 1

  • Transplanting of colonies and microscopic analysis.
  • Prepare tubes for sequencing :
    • HfsG
    • HfsH
    • HfsH + Ter
    • HfsG + HfsH + Ter
    • Promoter + RBS + RFP
    • PRR + GHT
    • I ccdA
    • CcdA F Y

System 3

  • Extraction of plasmids tubes 4 and 5.
  • Digestion and ligation of Quorum Sensing brick.

System 2

  • Transplanting ligations 9-10.
  • ligation of tube 4 with tube 7 to test if c2P22 well represses K145150.