ULB/12 July 2009

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Contents

July 12, 2009

Forward and reverse primers are designed and ordered.

Primers design for hfsG et hfsH genes

BBa_R0011 (promoter) :

PREFIX :

=> GAATTCATGAGATCT aattgtgagcggataaca

SUFFIX :

<= ctcgagttaggatcc TGTGCTCAGTATCTTGTT

RBS BBa_B0034 :

PREFIX :

=> GAATTCATGAGATCT AAAGAG

SUFFIX :

<= ctcgagttaggatcc TTTCTC

hfsG gene :

PREFIX :

=> GAATTCATGAGATCT atgaacgcgcccgtcaacgagctgcgactggagaacgccg

SUFFIX :

<= cagacggcctcgctgtaga

hfsH gene :

PREFIX :

=> atgccgatggagttcgagaag

SUFFIX :

<= ctcgagttaggatcc ttagagcccgatcc

RBS + RFP :

PREFIX :

=> GAATTCATGAGATCT aaagaggagaaatactag

SUFFIX :

<= ctcgagttaggatcc GCGATCTACACTAGCACT

BBa_B0015 terminator :

PREFIX :

=> GAATTCATGAGATCT ccaggcatcaaataaaac

SUFFIX :

<= ctcgagttaggatcc TATAAACGCAGAAAGGCC

Sequences

Promoter (55 bp)

aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca

RBS (12 pb)

aaagaggagaaa

RBS + RFP (681 bp)

aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgt
atggaaggttccgttaacggtcacgagttcgAaatcgaaggtgaaggtgaaggtcgtccgtacgaaggt
acccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcc
ttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccag
gactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt
gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaa
accacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatc
acctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggt
gcttaataacgctgatagtgctagtgtagatcgc

Terminator (129 bp)

ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata

Plasmid (3165 bp)

ggatcctaactcgaggttacattgtcgatctgttcatggtgaacagctttaaatgcaccaaaaactcgt
aaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgat
atctaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagcc
ataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgag
ccatgagaacgaaccattgagatcatgcttactttgcatgtcactcaaaaattttgcctcaaaactggt
gagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctga
tgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatc
catttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaa
tttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaa
ctcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgcctt
gtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaa
agacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttc
actaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcc
tttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacacc
ataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttc
tttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatg
ctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatct
caattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtcct
tttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctag
accctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatag
aataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtc
agttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccc
taaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatca
ggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggg
gtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccg
tcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagc
agttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggct
aatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattc
tcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcatt
actggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacagttaccaatgcttaatcag
tgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagat
aactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcacc
ggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagttt
gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcag
ctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctcctt
cggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgca
taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt
ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccaca
tagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttacc
gctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcac
cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaa
atgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgag
cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccatggtgcc
acctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaa
tttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcatgagatct