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ULB/14 August 2009
From 2009.igem.org
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| - | == | + | <html xmlns="http://www.w3.org/1999/xhtml"> |
| + | |||
| + | <head> | ||
| + | <meta http-equiv="content-type" content="text/html;charset=utf-8" /> | ||
| + | <title>iGEM Team:ULB-Brussels Wiki</title> | ||
| + | <link rel="stylesheet" type="text/css" href="style.css" media="screen" /> | ||
| + | <link rel="stylesheet" type="text/css" href="menu.css" media="screen" /> | ||
| + | |||
| + | <!-- Auto-Reload script for Dev --> | ||
| + | <script language="javascript" type="text/javascript"> | ||
| + | <!-- | ||
| + | function timedRefresh(timeoutPeriod) { | ||
| + | setTimeout("location.reload(true);",timeoutPeriod); | ||
| + | } | ||
| + | // --> | ||
| + | </script> | ||
| + | <style type="text/css"> | ||
| + | /* Design for https://2009.igem.org/Team:ULB-Brussels | ||
| + | |||
| + | Université Libre de Bruxelles | ||
| + | */ | ||
| + | |||
| + | html body { | ||
| + | width: 910px; | ||
| + | font-family: Arial; | ||
| + | margin: 0 auto; | ||
| + | |||
| + | } | ||
| + | |||
| + | html body div.header { | ||
| + | margin: 0px; | ||
| + | padding: 0px; | ||
| + | margin-top: -21px; /* strange... */ | ||
| + | } | ||
| + | |||
| + | /* ===== banner ===== */ | ||
| + | |||
| + | /* banner = large image on the top of the page, begind the title */ | ||
| + | |||
| + | html body div.header div.banner { | ||
| + | height: 171px; | ||
| + | background-image: url(https://static.igem.org/mediawiki/2009/0/0a/Logoulb1.png); | ||
| + | background-repeat: no-repeat; | ||
| + | } | ||
| + | |||
| + | html body div.header div.banner h1 { | ||
| + | padding-top: 50px; | ||
| + | |||
| + | text-align: center; | ||
| + | text-decoration: none; | ||
| + | border: none; | ||
| + | |||
| + | color: #4E7DB7; | ||
| + | font-size: 40px; | ||
| + | } | ||
| + | |||
| + | html body div.header ul { | ||
| + | /* these are common to both 'menu' and 'submenu' */ | ||
| + | margin-top: 0px; | ||
| + | margin-bottom: 0px; | ||
| + | |||
| + | list-style-type: none; | ||
| + | list-style-image: none; | ||
| + | } | ||
| + | |||
| + | /* ===== menu ===== */ | ||
| + | |||
| + | /* menu = horizontal bar containing the main links to other sections of the wiki */ | ||
| + | |||
| + | html body div.header div.menu { | ||
| + | background-color: #3E6DA4; | ||
| + | color: white; | ||
| + | |||
| + | height: 30px; | ||
| + | padding: 0px; | ||
| + | } | ||
| + | |||
| + | html body div.header div.menu li { | ||
| + | float: left; | ||
| + | text-align: center; | ||
| + | |||
| + | width: 120px; | ||
| + | margin: 0px; | ||
| + | margin-top: 10px; | ||
| + | } | ||
| + | |||
| + | html body div.header div.menu li :hover { | ||
| + | color: #5F7EB4; | ||
| + | background-color: #FFFFFF; | ||
| + | |||
| + | margin: 0px; | ||
| + | |||
| + | padding-left: 15px; | ||
| + | padding-right: 15px; | ||
| + | padding-top: 4px; | ||
| + | padding-bottom: 3px; | ||
| + | } | ||
| + | |||
| + | /* class 'selection' refers to the 'selected' menu, in order to change it's color to white */ | ||
| + | html body div.header div.menu li a.selection { | ||
| + | color: #5F7EB4; | ||
| + | background-color: #FFFFFF; | ||
| + | |||
| + | margin: 0px; | ||
| + | |||
| + | padding-left: 15px; | ||
| + | padding-right: 15px; | ||
| + | padding-top: 4px; | ||
| + | padding-bottom: 3px; | ||
| + | } | ||
| + | |||
| + | html body div.header div.menu li a { | ||
| + | color: white; | ||
| + | text-decoration: none; | ||
| + | } | ||
| + | |||
| + | /* ===== submenu ===== */ | ||
| + | |||
| + | /* submenu = changing horizontal bar containing the main links to subsections of other sections of the wiki */ | ||
| + | |||
| + | html body div.header div.submenu { | ||
| + | /*background-color: #404040;*/ | ||
| + | background-color: white; | ||
| + | height: 25px; | ||
| + | |||
| + | font-size: small; | ||
| + | |||
| + | border-bottom-style: solid; | ||
| + | border-bottom-width: 1px; | ||
| + | border-bottom-color: #AAAAAA; | ||
| + | } | ||
| + | |||
| + | html body div.header div.submenu li { | ||
| + | float: left; | ||
| + | width: 120px; | ||
| + | text-align: center; | ||
| + | |||
| + | margin: 0px; | ||
| + | margin-top: 4px; | ||
| + | } | ||
| + | |||
| + | html body div.header div.submenu li :hover { | ||
| + | color: #5F7EB4; | ||
| + | background-color: #FFFFFF; | ||
| + | |||
| + | margin: 0px; | ||
| + | |||
| + | padding-left: 15px; | ||
| + | padding-right: 15px; | ||
| + | padding-bottom: 3px; | ||
| + | } | ||
| + | |||
| + | html body div.header div.submenu li a { | ||
| + | color: #5F7EB4; | ||
| + | text-decoration: none; | ||
| + | } | ||
| + | |||
| + | /* ===== panel ===== */ | ||
| + | |||
| + | /* panel = box containing mascotte, contdown and links to sponsors */ | ||
| + | |||
| + | html body div.panel { | ||
| + | float: right; | ||
| + | border-left-style: solid; | ||
| + | border-left-width: 1px; | ||
| + | border-left-color: #AAAAAA; | ||
| + | |||
| + | padding-left: 10px; | ||
| + | margin-left: 10px; | ||
| + | margin-top: 20px; | ||
| + | |||
| + | width: 250px; | ||
| + | font-size: small; | ||
| + | } | ||
| + | |||
| + | /* appearance of the links */ | ||
| + | html body div.panel a { | ||
| + | text-decoration: none; | ||
| + | font-weight: bold; | ||
| + | color: #3E6DA4; | ||
| + | } | ||
| + | |||
| + | html body div.panel a:hover { | ||
| + | color: #F5B455; | ||
| + | } | ||
| + | |||
| + | /* == countdown == */ | ||
| + | |||
| + | html body div.panel div.countdown { | ||
| + | /*margin-top: 20px; | ||
| + | margin-bottom: 20px; | ||
| + | height: 30px;*/ | ||
| + | margin: 20px; | ||
| + | padding: 20px; | ||
| + | } | ||
| + | |||
| + | .countdown font { | ||
| + | padding: 20px; | ||
| + | margin: 20px; | ||
| + | color: #5F7EB4; | ||
| + | } | ||
| + | |||
| + | /* == sponsors == */ | ||
| + | |||
| + | html body div.panel div.sponsor { | ||
| + | margin-bottom: 10px; | ||
| + | min-height: 50px; | ||
| + | } | ||
| + | |||
| + | /* appearance of the sponsor's logos */ | ||
| + | html body div.panel div.sponsor img { | ||
| + | width: 50px; | ||
| + | height: 50px; | ||
| + | border-style: none; | ||
| + | float: left; | ||
| + | margin-right: 7px; | ||
| + | } | ||
| + | |||
| + | /* ===== main ===== */ | ||
| + | |||
| + | html body div.main { | ||
| + | font-size: small; | ||
| + | padding-left: 10px; | ||
| + | padding-right: 10px; | ||
| + | width: 910; | ||
| + | text-align: justify; | ||
| + | } | ||
| + | |||
| + | html body div.main p.abstract { | ||
| + | font-weight: bold; | ||
| + | } | ||
| + | |||
| + | html body div.main div.welcome img { | ||
| + | width: 626px; | ||
| + | height: 150px; | ||
| + | margin-top: 10px; | ||
| + | border-style: none; | ||
| + | } | ||
| + | |||
| + | html body div.footer { | ||
| + | } | ||
| + | |||
| + | </style> | ||
| + | |||
| + | </head> | ||
| + | |||
| + | <!-- *** Uncomment next line to enable autorefresh (developper mode) *** --> | ||
| + | <!-- <body onload="JavaScript:timedRefresh(5000);"> --> | ||
| + | <!-- <body> --> | ||
| + | <body> | ||
| + | <!-- | ||
| + | header : box on the top of the page containing the banner and links to sections and subsections of | ||
| + | the wiki. | ||
| + | --> | ||
| + | <div class="header"> | ||
| + | |||
| + | <div class="banner"> | ||
| + | <h1></h1> | ||
| + | </div> | ||
| + | <div class="menu"> | ||
| + | <ul> | ||
| + | <li><a class="mainlink" href="/Team:ULB-Brussels">Home</a></li> | ||
| + | <li><a class="mainlink" href="/Team:ULB-Brussels/Team">Team</a></li> | ||
| + | <li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | ||
| + | <li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | ||
| + | <li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li> | ||
| + | <li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | ||
| + | <li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | <div class="submenu"> | ||
| + | <ul> | ||
| + | <!-- | ||
| + | <li><a href="#english">English</a></li> | ||
| + | <li><a href="#french">French</a></li> | ||
| + | |||
| + | |||
| + | <li><a href="#dutch">Dutch</a></li> | ||
| + | <li><a href="#german">German</a></li> | ||
| + | <li><a href="#spanish">Spanish</a></li> | ||
| + | <li><a href="#italian">Italiano</a></li> | ||
| + | <li><a href="#chinese">Chinese</a></li> | ||
| + | --> | ||
| + | |||
| + | |||
| + | </ul> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <!-- | ||
| + | panel : box on the right containing a countdown, links to the sponsors | ||
| + | |||
| + | [ REMOVED ] | ||
| + | --> | ||
| + | |||
| + | <!-- | ||
| + | main : main part of the page, containing the text | ||
| + | --> | ||
| + | <div class="main"> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="footer"> | ||
| + | </div> | ||
| + | |||
| + | </body> | ||
| + | </html> | ||
| + | |||
| + | |||
| + | <html> | ||
| + | <style type="text/css"> | ||
| + | |||
| + | |||
| + | |||
| + | table.calendar { margin: 0; padding: 10px; } | ||
| + | table.calendar td { margin: 0; padding: 2px; vertical-align: top; } | ||
| + | table.month .heading td { padding:2px; background-color:#3E6DA4; color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } | ||
| + | table.month .dow td { color:#aaa; text-align:center; font-size:110%; } | ||
| + | table.month td.today { background-color:#ddd; } | ||
| + | table.month td { | ||
| + | border: none; | ||
| + | margin: 0; | ||
| + | padding: 1pt 1.5pt; | ||
| + | font-weight: bold; | ||
| + | font-size: 12pt; | ||
| + | text-align: right; | ||
| + | background-color: #eee; | ||
| + | } | ||
| + | #bodyContent table.month a { background:none; padding:0 } | ||
| + | .day-active { color:blue } | ||
| + | .day-empty { color:grey } | ||
| + | |||
| + | |||
| + | </style> | ||
| + | </html> | ||
| + | |||
| + | {| align="right" | ||
| + | {| style="color:#0c6;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#035e7b" align="right" | ||
| + | |||
| + | !align="right"|{{#calendar: title=ULB |year=2009 | month=07}} | ||
| + | |- | ||
| + | !align="right"|{{#calendar: title=ULB |year=2009 | month=08}} | ||
| + | |- | ||
| + | !align="right"|{{#calendar: title=ULB |year=2009 | month=09}} | ||
| + | |||
| + | |} | ||
| + | |||
A glycerol is ready for bacteria having integrated the RBS. | A glycerol is ready for bacteria having integrated the RBS. | ||
Latest revision as of 10:43, 21 October 2009
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A glycerol is ready for bacteria having integrated the RBS.
Plasmids containing the RBS have been purified using the purification site (2x 1500µl of culture). 7µl of each purification were digested with 1µl of XbaI, PstI and 1µl of tampon H.
An electrophoresis on gel confirmed the presence of the plasmids in each tube. Parasites on the first tube are probably due to an incomplete digestion.
The quantity of purified plasmids was evaluated using a photometry analysis on a 100x dilution.
All plasmids show a concentration close to 50ng and a very acceptable purity.
hfsG and hfsH have been re-suspended in water (50µl) and then diluted 20 times.
The ligation of the promoter and the RBS in the destination plasmid have been stopped by freezing those at 80°C during 20 min.
Bacteria have been transformed with hfsG, hfsH (to keep a stock of plasmids) and our 2 ligations (in order to check wether it did work or not).
