ULB/1 September 2009

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iGEM Team:ULB-Brussels Wiki

Colonies containing promoter + RBS + RFP gave some other colonies:

  • Rose colonies: the promoter became constitutive or it strongly expresses, but when you extract the plasmids and do migrations after digestion with EcorRI and PstI, no bands is visible.
  • White colonies: the promoter hasn't mutated.