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ULB/22 July 2009
From 2009.igem.org
(Difference between revisions)
| Line 376: | Line 376: | ||
</table> | </table> | ||
| - | Running on a agarose gel (55° and | + | Running on a agarose gel (55° and 60° programs) |
=== Results === | === Results === | ||
* biobricks : ok | * biobricks : ok | ||
* hfsG and hfsH gene: same results as previously | * hfsG and hfsH gene: same results as previously | ||
Revision as of 09:24, 5 October 2009
Amplification
PCR on:
- hfsG gene
- hfsH gene
- promotor
- terminator
- RBS + RFP
PCR cycles
3 different PCR cycles have been carried out :
- at 55°C
- at 60°C
- a special « deletion programme» (for longer primers)
| Temperature (°C) | DMSO | DMSO | No DMSO | No DMSO |
|---|---|---|---|---|
| 55 | ADN | Control | ADN | Control |
| 60 | ADN | Control | ADN | Control |
| deletion | ADN | Control | ADN | Control |
Running on a agarose gel (55° and 60° programs)
Results
- biobricks : ok
- hfsG and hfsH gene: same results as previously
