From 2009.igem.org
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Revision as of 09:23, 5 October 2009
iGEM Team:ULB-Brussels Wiki
Amplification
PCR on:
- hfsG gene
- hfsH gene
- promotor
- terminator
- RBS + RFP
PCR cycles
3 different PCR cycles have been carried out :
- at 55°C
- at 60°C
- a special « deletion programme» (for longer primers)
| Temperature (°C) |
DMSO |
DMSO |
No DMSO |
No DMSO |
| 55 |
ADN |
Control |
ADN |
Control |
| 60 |
ADN |
Control |
ADN |
Control |
| deletion |
ADN |
Control |
ADN |
Control |
Running on a agarose gel (55° and 6O° programs)
Results
- biobricks : ok
- hfsG and hfsH gene: same results as previously
"
