ULB/23 July 2009

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iGEM Team:ULB-Brussels Wiki

July 23, 2009

Results for the deletion program after running on a gel :

  • we get a band for the hfsG gene with DMSO. Parasite bands are very light.
  • hfsH gene: still lots of parasite bands…

Discussion on the PCR programs.

Maybe we should increase the annealing temperature or dilute the mix hfsH gene with DMSO ?

Gradient PCR on promoter : 44 to 56°C

Miniprep of bacterial culture to extract the assembly standart 21.

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