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ULB/24 July 2009
From 2009.igem.org
July 24, 2009
No pertinent result emerged from the gradient PCR.
RBS primers hybridisation
A restriction digest was performed to cut the plasmid with EcoRI and XhoI.
PCR (deletion program) on hfsH gene at 68°C with and without DMSO (the mix has been 10 times diluted).
hfsH and hfsG genes have been ordered for the assembly standard 10.
