ULB/25 August 2009

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(Difference between revisions)
(New page: == August 25, 2009 == <div style='color:grey;'> Extraction de hfsH + terminateur et de ccdA reverse Résultats : *3eme puis à 870pb correspond à hfsH +terminateur *ccdA : bande pas trè...)
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== August 25, 2009 ==
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<div style='color:grey;'>
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Extraction de hfsH + terminateur  et de ccdA reverse
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Extraction of hFSH + terminator and reverse ccdA
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Résultats :
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*3eme puis à 870pb correspond à hfsH +terminateur
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Results:
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*ccdA : bande pas très visible  
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*the 3rd then the 870th base pair corresponds to hFSH + terminator
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*ccdA: not very visible band
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[[Image:25_7.jpg]]
[[Image:25_7.jpg]]
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Ligation de hfsG et hfsH + terminateur sur le plasmide contenant hfsG résistant à l’ ampicilline.
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Ligation of hfsG and hFSH + terminator on the plasmid containing hfsG resistant to the ampicillin. Bacterial culture to ascertain the presence of RBS + RFP
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Culture de bactéries pour s’assurer de la présence de RBS+RFP
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</div>
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Revision as of 20:09, 30 September 2009


Extraction of hFSH + terminator and reverse ccdA

Results:

  • the 3rd then the 870th base pair corresponds to hFSH + terminator
  • ccdA: not very visible band

25 7.jpg

Ligation of hfsG and hFSH + terminator on the plasmid containing hfsG resistant to the ampicillin. Bacterial culture to ascertain the presence of RBS + RFP

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