ULB/25 August 2009
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(New page: == August 25, 2009 == <div style='color:grey;'> Extraction de hfsH + terminateur et de ccdA reverse Résultats : *3eme puis à 870pb correspond à hfsH +terminateur *ccdA : bande pas trè...) |
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- | Extraction | + | Extraction of hFSH + terminator and reverse ccdA |
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- | * | + | Results: |
- | *ccdA : | + | |
+ | *the 3rd then the 870th base pair corresponds to hFSH + terminator | ||
+ | *ccdA: not very visible band | ||
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[[Image:25_7.jpg]] | [[Image:25_7.jpg]] | ||
- | Ligation | + | Ligation of hfsG and hFSH + terminator on the plasmid containing hfsG resistant to the ampicillin. Bacterial culture to ascertain the presence of RBS + RFP |
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Revision as of 20:09, 30 September 2009
Extraction of hFSH + terminator and reverse ccdA
Results:
- the 3rd then the 870th base pair corresponds to hFSH + terminator
- ccdA: not very visible band
Ligation of hfsG and hFSH + terminator on the plasmid containing hfsG resistant to the ampicillin. Bacterial culture to ascertain the presence of RBS + RFP