ULB/29 July 2009

From 2009.igem.org

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== July 29, 2009 ==
 
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<div style='color:grey;'>
 
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PCR (programme délétion) de RBS+Promoteur (avec et sans DMSO) en utilisant l’amorce forward du promoteur et l’amorce reverse du RBS<br/>
 
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PCR des gènes hfsH et hfsG avec les nouvelles amorces<br/>
 
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Migration sur gel du plasmide purifié. <br/> <br/> Résultat : bande d’intérêt mince<br/>
 
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[[Image:29_7.jpg]]
 
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</div>
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PCR (deletion program) by RBS + promoter (with and without DMSO) using the forward primer of promoter and reverse primer of RBS.
 +
 
 +
PCR of hFSH and hfsG with new primers.
 +
 
 +
Gel migration of purified plasmid.
 +
 
 +
Result: band of interest is thin.

Revision as of 17:35, 20 September 2009

PCR (deletion program) by RBS + promoter (with and without DMSO) using the forward primer of promoter and reverse primer of RBS.

PCR of hFSH and hfsG with new primers.

Gel migration of purified plasmid.

Result: band of interest is thin.