ULB/2 July 2009

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(New page: Report iGEM of July 1, 2009 We began the first morning by a recall presentation from the principles of molecular biology by Gilles our advisor. He also pointed out us the various tools ...)
 
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Report iGEM of July 1, 2009
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We began the first morning by a recall presentation from the principles of molecular biology by Gilles our advisor.
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<title>iGEM Team:ULB-Brussels Wiki</title>
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  He also pointed out us the various tools of molecular biology which we will have probably to use during handling:
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•        PCR,
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    Université Libre de Bruxelles
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•        Synthesis/sequencing of DNA,
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•        Microscopy, etc
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    It will be useful for us also to reflect, in the case of our subject, for the excretion of our product, with one or the other sequence signal, with resistances to antibiotics, a possible system poison-antidote,…
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All that depending obviously on the way in which will arrange the project.
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Our frame is : Escherichia coli.
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With regard to the prices decreed for the contest, to obtain a certain medal, it is necessary to carry out a certain number of tasks; in short (for more details: https://2009.igem.org/Judging/Judging_Criteria):
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For a bronze medal, it is necessary:
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• To fill a report/ratio of project
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·        To create a wiki
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·        Te create  a poster and to present the project orally
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·        To imagine a new part Biobrick (theoretical point of view) with informing well/describing! (note: PoPS = polymerase a second, the measuring unit)
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For a money medal, it is necessary:
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·        To prouve that it walk
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·          To characterize a new part (manipulation point of view of)
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•        To characterize or improve an already existing part
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•        To help another team
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In addition to these medals, there are different other prices detailed here. One also speaks about “under-competitions” among which it is necessary to choose in which field between our subject.
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A priori, our project is part of  in the category “Best manufacturing project”.
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For the absents : this page gives very full with super information interesting, it would be well to take time to read the contents of it to have the clearer ideas for certain things and according to your basic training.
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At the end of the morning and until the following day afternoon, we carried out some basic handling of molecular biology which will be more useful to us in the continuation of the project:
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•        Insertion, by electroporation, of plasmides for the fluorescent protein expression (RFP, GFP.) in E. coli (= transformation) + visualization under the microscope. PCR + freezing of agarose
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•        Extraction of DNA + restriction with one or two enzymes + migration on freezing of agarose.
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In end-of-day of Thursday, meeting to decide tasks to make soon, essentially biblio, to see list written by Hugo. Each one is held to get information about a little all that was defined, by making a SORTING of information before subjecting it to all so that one is not drowned under the mass of information.
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A meeting for pooling and continuation of the project is envisaged Wednesday July 8 with 8:00 with the library of Solbosh.
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Lastly, a calendar was also drawn up in order to visualize which leaves when on holiday and/or works and/or has one second session.
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Molecular biology and Cell Biology Cellular reminders.
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Choice of frame: '''''Escherichia coli'''''.
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*Brainstorming.
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*Focusing on biofilms and especially on ''Caulobacter crescentus'' glue biosynthesis pathway.
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'''Purpose''' : to transfer the Caulobacter crescentus holdfast polysaccharide
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biosynthesis pathway to E. Coli. The holdfast is an elactic and strongly adhesive material with gel-like properties1.

Latest revision as of 21:53, 20 October 2009

iGEM Team:ULB-Brussels Wiki


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Molecular biology and Cell Biology Cellular reminders.

Choice of frame: Escherichia coli.


  • Brainstorming.
  • Focusing on biofilms and especially on Caulobacter crescentus glue biosynthesis pathway.


Purpose : to transfer the Caulobacter crescentus holdfast polysaccharide biosynthesis pathway to E. Coli. The holdfast is an elactic and strongly adhesive material with gel-like properties1.

Retrieved from "http://2009.igem.org/ULB/2_July_2009"