ULB/3 September 2009

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iGEM Team:ULB-Brussels Wiki

Ligation and transformation (promoter-RBS-RFP and hfsG-hFSH): Results: Positive

Extraction of plasmids and verification of the presence of bricks: Results: negative

Cultivation in IPTG medium with the pink bacteria with promoter-RBS-RFP and other (white one) also with promoter-RBS-RFP. Results:

  • Pink colonies: positive (fluorescence)
  • White colonies: negative (no fluorescence)

Transformation with following plasmids plates:

  • lux pR R0062
  • Cl434 R1052,
  • P0152
  • K145150
  • luxI C0061,
  • Cllp22R0053,
  • E0240,
  • LuxR I0462