ULB/4 August 2009

From 2009.igem.org

(Difference between revisions)

Revision as of 17:28, 20 September 2009

laboratory manipulation

Purification of hfsG.

Analysis of hFSH: we notice that the primers can bind to 25 different locations in the genome of Caulobacter. We will digest the genome with a maximum of possible sites to better identify the hFSH (selection of a restriction enzymes which don't cut hFSH) before doing a PCR with "Blunt" primers.

@@ Line 1: / Line 1: @@
-== August 4, 2009 ==
 ===laboratory manipulation===
-<div style='color:grey;'>
-Purification de hfsG.
+Purification of hfsG.
-Analyse du gène hfsH: on remarque que les amorces peuvent se lier à 25 endroits différents dans le génome de Caulobacter. On va digérer le génome avec un maximum de sites possible pour mieux cerner le gène hfsH (sélection des enzymes de restriction ne coupant pas hfsH) avant de faire un PCR avec les amorces « Blunt »
-</div>
+Analysis of hFSH: we notice that the primers can bind to 25 different locations in the genome of Caulobacter. We will digest the genome with a maximum of possible sites to better identify the hFSH (selection of a restriction enzymes which don't cut hFSH) before doing a PCR with "Blunt" primers.