ULB/4 August 2009

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laboratory manipulation

Purification of hfsG.

Analysis of hFSH: we notice that the primers can bind to 25 different locations in the genome of Caulobacter. We will digest the genome with a maximum of possible sites to better identify the hFSH (selection of a restriction enzymes which don't cut hFSH) before doing a PCR with "Blunt" primers.