ULB/5 September 2009
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=> Résultats (voir photo ci-dessous): négatifs pour les genes : luxPr (R0062), P0152, K14150, cII p22 (R0053), les autres sont positifs. | => Résultats (voir photo ci-dessous): négatifs pour les genes : luxPr (R0062), P0152, K14150, cII p22 (R0053), les autres sont positifs. | ||
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+ | Positive results for bacterial transformations performed on 9/4. | ||
+ | Extraction and migration of plasmids with following genes: | ||
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+ | *1st well: ''LuxPr (R0062)'' | ||
+ | *2nd well: ''P0152'' | ||
+ | *3th well: ''cl 434 (R0052)'' | ||
+ | *4th well: ''promoter repressed by cIIp22 and activated by HSL-LuxR (K14150)'' | ||
+ | *5th well: ''cII p22 (R0053)'' | ||
+ | *6th well: ''GFP (e0240)'' | ||
+ | *7th well: ''luxR (I0462)'' | ||
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+ | => Results (see below): negative for: ''luxPr (R0062), P0152, K14150, cII p22 (R0053)'', others are positive. | ||
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[[Image:5_9.jpg]] | [[Image:5_9.jpg]] | ||
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- | Ligation | + | Ligation: |
{| class="wikitable " style="text-align:center; width:80%;"cellpadding="3" cellspacing="1" border="1" | {| class="wikitable " style="text-align:center; width:80%;"cellpadding="3" cellspacing="1" border="1" | ||
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Revision as of 21:30, 30 September 2009
=> Résultats (voir photo ci-dessous): négatifs pour les genes : luxPr (R0062), P0152, K14150, cII p22 (R0053), les autres sont positifs.
Positive results for bacterial transformations performed on 9/4. Extraction and migration of plasmids with following genes:
- 1st well: LuxPr (R0062)
- 2nd well: P0152
- 3th well: cl 434 (R0052)
- 4th well: promoter repressed by cIIp22 and activated by HSL-LuxR (K14150)
- 5th well: cII p22 (R0053)
- 6th well: GFP (e0240)
- 7th well: luxR (I0462)
=> Results (see below): negative for: luxPr (R0062), P0152, K14150, cII p22 (R0053), others are positive.
Ligation:
N° | Insert | Plasmid |
---|---|---|
1 |
| |
2 | ||
3 | ||
4 | LuxpR | |
5 |
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