ULB/5 September 2009

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iGEM Team:ULB-Brussels Wiki


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Positive results for bacterial transformations performed on 9/4. Extraction and migration of plasmids with following genes:

  • 1st well: LuxPr (R0062)
  • 2nd well: P0152
  • 3th well: cl 434 (R0052)
  • 4th well: promoter repressed by cIIp22 and activated by HSL-LuxR (K14150)
  • 5th well: cII p22 (R0053)
  • 6th well: GFP (e0240)
  • 7th well: luxR (I0462)

=> Results (see below): negative for: luxPr (R0062), P0152, K14150, cII p22 (R0053), others are positive.

5 9.jpg

Ligation:

Insert Plasmid
1


2
3
4 LuxpR
5