ULB/7 September 2009
From 2009.igem.org
(Difference between revisions)
Line 335: | Line 335: | ||
</style> | </style> | ||
</html> | </html> | ||
+ | |||
+ | {| align="right" | ||
+ | {| style="color:#0c6;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#035e7b" align="right" | ||
+ | |||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=07}} | ||
+ | |- | ||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=08}} | ||
+ | |- | ||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=09}} | ||
+ | |||
+ | |} | ||
+ | |||
Results of ligation: | Results of ligation: | ||
Revision as of 09:01, 15 October 2009
| ||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ||||||||||||||||||||||||||||||||||||||||||||||||||
|
Results of ligation:
- Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
- Phenotypic tests:
- Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.
- Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.
Extracting of plasmides 9, 10, 5, 6 and 2.
Transformation of 5 ligations carried out on 9/5.
Dubbing of the ligation 1 with IPTG in IPTG medium.
- Results:No fluorescence
Spreading of a large quantity of ligation 1 and 2. Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.
Following ligations:
- LIG1: Tube 4 (insert) + Tube 5
- Lig2: Tube 5 (insert) + Tube 9