ULB/7 September 2009
From 2009.igem.org
(Difference between revisions)
(5 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | == | + | <html xmlns="http://www.w3.org/1999/xhtml"> |
- | + | ||
- | + | <head> | |
- | *Colonies | + | <meta http-equiv="content-type" content="text/html;charset=utf-8" /> |
- | * | + | <title>iGEM Team:ULB-Brussels Wiki</title> |
- | ** | + | <link rel="stylesheet" type="text/css" href="style.css" media="screen" /> |
- | **Comparaison | + | <link rel="stylesheet" type="text/css" href="menu.css" media="screen" /> |
- | + | ||
- | Transformation | + | <!-- Auto-Reload script for Dev --> |
- | + | <script language="javascript" type="text/javascript"> | |
- | * | + | <!-- |
- | + | function timedRefresh(timeoutPeriod) { | |
- | Ligation | + | setTimeout("location.reload(true);",timeoutPeriod); |
- | + | } | |
- | # | + | // --> |
- | #Lig2 : Tube 5 (insert) + Tube 9 | + | </script> |
- | + | <style type="text/css"> | |
+ | /* Design for https://2009.igem.org/Team:ULB-Brussels | ||
+ | |||
+ | Université Libre de Bruxelles | ||
+ | */ | ||
+ | |||
+ | html body { | ||
+ | width: 910px; | ||
+ | font-family: Arial; | ||
+ | margin: 0 auto; | ||
+ | |||
+ | } | ||
+ | |||
+ | html body div.header { | ||
+ | margin: 0px; | ||
+ | padding: 0px; | ||
+ | margin-top: -21px; /* strange... */ | ||
+ | } | ||
+ | |||
+ | /* ===== banner ===== */ | ||
+ | |||
+ | /* banner = large image on the top of the page, begind the title */ | ||
+ | |||
+ | html body div.header div.banner { | ||
+ | height: 171px; | ||
+ | background-image: url(https://static.igem.org/mediawiki/2009/0/0a/Logoulb1.png); | ||
+ | background-repeat: no-repeat; | ||
+ | } | ||
+ | |||
+ | html body div.header div.banner h1 { | ||
+ | padding-top: 50px; | ||
+ | |||
+ | text-align: center; | ||
+ | text-decoration: none; | ||
+ | border: none; | ||
+ | |||
+ | color: #4E7DB7; | ||
+ | font-size: 40px; | ||
+ | } | ||
+ | |||
+ | html body div.header ul { | ||
+ | /* these are common to both 'menu' and 'submenu' */ | ||
+ | margin-top: 0px; | ||
+ | margin-bottom: 0px; | ||
+ | |||
+ | list-style-type: none; | ||
+ | list-style-image: none; | ||
+ | } | ||
+ | |||
+ | /* ===== menu ===== */ | ||
+ | |||
+ | /* menu = horizontal bar containing the main links to other sections of the wiki */ | ||
+ | |||
+ | html body div.header div.menu { | ||
+ | background-color: #3E6DA4; | ||
+ | color: white; | ||
+ | |||
+ | height: 30px; | ||
+ | padding: 0px; | ||
+ | } | ||
+ | |||
+ | html body div.header div.menu li { | ||
+ | float: left; | ||
+ | text-align: center; | ||
+ | |||
+ | width: 120px; | ||
+ | margin: 0px; | ||
+ | margin-top: 10px; | ||
+ | } | ||
+ | |||
+ | html body div.header div.menu li :hover { | ||
+ | color: #5F7EB4; | ||
+ | background-color: #FFFFFF; | ||
+ | |||
+ | margin: 0px; | ||
+ | |||
+ | padding-left: 15px; | ||
+ | padding-right: 15px; | ||
+ | padding-top: 4px; | ||
+ | padding-bottom: 3px; | ||
+ | } | ||
+ | |||
+ | /* class 'selection' refers to the 'selected' menu, in order to change it's color to white */ | ||
+ | html body div.header div.menu li a.selection { | ||
+ | color: #5F7EB4; | ||
+ | background-color: #FFFFFF; | ||
+ | |||
+ | margin: 0px; | ||
+ | |||
+ | padding-left: 15px; | ||
+ | padding-right: 15px; | ||
+ | padding-top: 4px; | ||
+ | padding-bottom: 3px; | ||
+ | } | ||
+ | |||
+ | html body div.header div.menu li a { | ||
+ | color: white; | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | |||
+ | /* ===== submenu ===== */ | ||
+ | |||
+ | /* submenu = changing horizontal bar containing the main links to subsections of other sections of the wiki */ | ||
+ | |||
+ | html body div.header div.submenu { | ||
+ | /*background-color: #404040;*/ | ||
+ | background-color: white; | ||
+ | height: 25px; | ||
+ | |||
+ | font-size: small; | ||
+ | |||
+ | border-bottom-style: solid; | ||
+ | border-bottom-width: 1px; | ||
+ | border-bottom-color: #AAAAAA; | ||
+ | } | ||
+ | |||
+ | html body div.header div.submenu li { | ||
+ | float: left; | ||
+ | width: 120px; | ||
+ | text-align: center; | ||
+ | |||
+ | margin: 0px; | ||
+ | margin-top: 4px; | ||
+ | } | ||
+ | |||
+ | html body div.header div.submenu li :hover { | ||
+ | color: #5F7EB4; | ||
+ | background-color: #FFFFFF; | ||
+ | |||
+ | margin: 0px; | ||
+ | |||
+ | padding-left: 15px; | ||
+ | padding-right: 15px; | ||
+ | padding-bottom: 3px; | ||
+ | } | ||
+ | |||
+ | html body div.header div.submenu li a { | ||
+ | color: #5F7EB4; | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | |||
+ | /* ===== panel ===== */ | ||
+ | |||
+ | /* panel = box containing mascotte, contdown and links to sponsors */ | ||
+ | |||
+ | html body div.panel { | ||
+ | float: right; | ||
+ | border-left-style: solid; | ||
+ | border-left-width: 1px; | ||
+ | border-left-color: #AAAAAA; | ||
+ | |||
+ | padding-left: 10px; | ||
+ | margin-left: 10px; | ||
+ | margin-top: 20px; | ||
+ | |||
+ | width: 250px; | ||
+ | font-size: small; | ||
+ | } | ||
+ | |||
+ | /* appearance of the links */ | ||
+ | html body div.panel a { | ||
+ | text-decoration: none; | ||
+ | font-weight: bold; | ||
+ | color: #3E6DA4; | ||
+ | } | ||
+ | |||
+ | html body div.panel a:hover { | ||
+ | color: #F5B455; | ||
+ | } | ||
+ | |||
+ | /* == countdown == */ | ||
+ | |||
+ | html body div.panel div.countdown { | ||
+ | /*margin-top: 20px; | ||
+ | margin-bottom: 20px; | ||
+ | height: 30px;*/ | ||
+ | margin: 20px; | ||
+ | padding: 20px; | ||
+ | } | ||
+ | |||
+ | .countdown font { | ||
+ | padding: 20px; | ||
+ | margin: 20px; | ||
+ | color: #5F7EB4; | ||
+ | } | ||
+ | |||
+ | /* == sponsors == */ | ||
+ | |||
+ | html body div.panel div.sponsor { | ||
+ | margin-bottom: 10px; | ||
+ | min-height: 50px; | ||
+ | } | ||
+ | |||
+ | /* appearance of the sponsor's logos */ | ||
+ | html body div.panel div.sponsor img { | ||
+ | width: 50px; | ||
+ | height: 50px; | ||
+ | border-style: none; | ||
+ | float: left; | ||
+ | margin-right: 7px; | ||
+ | } | ||
+ | |||
+ | /* ===== main ===== */ | ||
+ | |||
+ | html body div.main { | ||
+ | font-size: small; | ||
+ | padding-left: 10px; | ||
+ | padding-right: 10px; | ||
+ | width: 910; | ||
+ | text-align: justify; | ||
+ | } | ||
+ | |||
+ | html body div.main p.abstract { | ||
+ | font-weight: bold; | ||
+ | } | ||
+ | |||
+ | html body div.main div.welcome img { | ||
+ | width: 626px; | ||
+ | height: 150px; | ||
+ | margin-top: 10px; | ||
+ | border-style: none; | ||
+ | } | ||
+ | |||
+ | html body div.footer { | ||
+ | } | ||
+ | |||
+ | </style> | ||
+ | |||
+ | </head> | ||
+ | |||
+ | <!-- *** Uncomment next line to enable autorefresh (developper mode) *** --> | ||
+ | <!-- <body onload="JavaScript:timedRefresh(5000);"> --> | ||
+ | <!-- <body> --> | ||
+ | <body> | ||
+ | <!-- | ||
+ | header : box on the top of the page containing the banner and links to sections and subsections of | ||
+ | the wiki. | ||
+ | --> | ||
+ | <div class="header"> | ||
+ | |||
+ | <div class="banner"> | ||
+ | <h1></h1> | ||
+ | </div> | ||
+ | <div class="menu"> | ||
+ | <ul> | ||
+ | <li><a class="mainlink" href="/Team:ULB-Brussels">Home</a></li> | ||
+ | <li><a class="mainlink" href="/Team:ULB-Brussels/Team">Team</a></li> | ||
+ | <li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | ||
+ | <li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | ||
+ | <li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li> | ||
+ | <li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | ||
+ | <li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <div class="submenu"> | ||
+ | <ul> | ||
+ | <!-- | ||
+ | <li><a href="#english">English</a></li> | ||
+ | <li><a href="#french">French</a></li> | ||
+ | |||
+ | |||
+ | <li><a href="#dutch">Dutch</a></li> | ||
+ | <li><a href="#german">German</a></li> | ||
+ | <li><a href="#spanish">Spanish</a></li> | ||
+ | <li><a href="#italian">Italiano</a></li> | ||
+ | <li><a href="#chinese">Chinese</a></li> | ||
+ | --> | ||
+ | |||
+ | |||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!-- | ||
+ | panel : box on the right containing a countdown, links to the sponsors | ||
+ | |||
+ | [ REMOVED ] | ||
+ | --> | ||
+ | |||
+ | <!-- | ||
+ | main : main part of the page, containing the text | ||
+ | --> | ||
+ | <div class="main"> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="footer"> | ||
+ | </div> | ||
+ | |||
+ | </body> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <style type="text/css"> | ||
+ | |||
+ | |||
+ | |||
+ | table.calendar { margin: 0; padding: 10px; } | ||
+ | table.calendar td { margin: 0; padding: 2px; vertical-align: top; } | ||
+ | table.month .heading td { padding:2px; background-color:#3E6DA4; color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } | ||
+ | table.month .dow td { color:#aaa; text-align:center; font-size:110%; } | ||
+ | table.month td.today { background-color:#ddd; } | ||
+ | table.month td { | ||
+ | border: none; | ||
+ | margin: 0; | ||
+ | padding: 1pt 1.5pt; | ||
+ | font-weight: bold; | ||
+ | font-size: 12pt; | ||
+ | text-align: right; | ||
+ | background-color: #eee; | ||
+ | } | ||
+ | #bodyContent table.month a { background:none; padding:0 } | ||
+ | .day-active { color:blue } | ||
+ | .day-empty { color:grey } | ||
+ | |||
+ | |||
+ | </style> | ||
+ | </html> | ||
+ | |||
+ | {| align="right" | ||
+ | {| style="color:#0c6;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#035e7b" align="right" | ||
+ | |||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=07}} | ||
+ | |- | ||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=08}} | ||
+ | |- | ||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=09}} | ||
+ | |||
+ | |} | ||
+ | |||
+ | Results of ligation: | ||
+ | |||
+ | * Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick. | ||
+ | |||
+ | * Phenotypic tests: | ||
+ | |||
+ | ** Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance. | ||
+ | |||
+ | ** Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste. | ||
+ | |||
+ | Extracting of plasmides 9, 10, 5, 6 and 2. | ||
+ | |||
+ | Transformation of 5 ligations carried out on 9/5. | ||
+ | |||
+ | Dubbing of the ligation 1 with IPTG in IPTG medium. | ||
+ | |||
+ | * Results:'''No fluorescence''' <br/> | ||
+ | Spreading of a large quantity of ligation 1 and 2. | ||
+ | Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator. | ||
+ | |||
+ | Following ligations: | ||
+ | # LIG1: Tube 4 (insert) + Tube 5 | ||
+ | # Lig2: Tube 5 (insert) + Tube 9 |
Latest revision as of 10:25, 21 October 2009
| ||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ||||||||||||||||||||||||||||||||||||||||||||||||||
|
Results of ligation:
- Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
- Phenotypic tests:
- Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.
- Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.
Extracting of plasmides 9, 10, 5, 6 and 2.
Transformation of 5 ligations carried out on 9/5.
Dubbing of the ligation 1 with IPTG in IPTG medium.
- Results:No fluorescence
Spreading of a large quantity of ligation 1 and 2. Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.
Following ligations:
- LIG1: Tube 4 (insert) + Tube 5
- Lig2: Tube 5 (insert) + Tube 9