ULB/7 September 2009

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== September 7, 2009 ==
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<html xmlns="http://www.w3.org/1999/xhtml">
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<div style='color:grey;'>
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Résultats de la ligation :
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<head>
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*Colonies de la ligation avec IPTG présentent un aspect filamenteux lorsqu’’ on pique avec un cure-dent.
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<meta http-equiv="content-type" content="text/html;charset=utf-8" />
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*Tests phénotypiques :
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<title>iGEM Team:ULB-Brussels Wiki</title>
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**Étalement des bactéries sur du verre et on a déposé une feuille de papier dessus : on peut sentir une résistance.
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<link rel="stylesheet" type="text/css" href="style.css" media="screen" />
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**Comparaison des billes d’ étalement issues des bactéries collante avec ceux d’ une autres transformation : les billes des bactéries collantes se sont collé et les billes témoins ne sont pas collé.
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<link rel="stylesheet" type="text/css" href="menu.css" media="screen" />
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Extraction des palsmides 9 , 10, 5, 6 et 2.<br/>
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Transformation des 5 ligations effectué le 5/9.<br/>
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<!-- Auto-Reload script for Dev -->
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Répiquage de la ligation 1 avec IPTG dans milieu avec IPTG<br/>
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<script language="javascript" type="text/javascript">
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*Résultats : '''Aucune fluorescence'''<br/>
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<!--
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Etalement d’ une grande quantité de ligation 1et2<br/>
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function timedRefresh(timeoutPeriod) {
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Ligation de promoteur-RBS-RFP et hfsG- hfsH-terminateur<br/>
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setTimeout("location.reload(true);",timeoutPeriod);
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Ligations suivante :  
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}
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#Lig1 : Tube 4 (insert) + Tube 5
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//  -->
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#Lig2 : Tube 5 (insert) + Tube 9
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</script>
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</div>
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{| align="right"
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{| style="color:#0c6;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#035e7b"  align="right"
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!align="right"|{{#calendar: title=ULB |year=2009 | month=07}}
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|-
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!align="right"|{{#calendar: title=ULB |year=2009 | month=08}}
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|-
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!align="right"|{{#calendar: title=ULB |year=2009 | month=09}}
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|}
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Results of ligation:
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* Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
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* Phenotypic tests:
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** Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.
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** Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.
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Extracting of plasmides 9, 10, 5, 6 and 2.
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Transformation of 5 ligations carried out on 9/5.
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Dubbing of the ligation 1 with IPTG in IPTG medium.
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* Results:'''No fluorescence''' <br/>  
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Spreading of a large quantity of ligation 1 and 2.
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Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.
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Following ligations:  
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# LIG1: Tube 4 (insert) + Tube 5  
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# Lig2: Tube 5 (insert) + Tube 9

Latest revision as of 10:25, 21 October 2009

iGEM Team:ULB-Brussels Wiki


July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30

Results of ligation:

  • Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
  • Phenotypic tests:
    • Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.
    • Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.

Extracting of plasmides 9, 10, 5, 6 and 2.

Transformation of 5 ligations carried out on 9/5.

Dubbing of the ligation 1 with IPTG in IPTG medium.

  • Results:No fluorescence

Spreading of a large quantity of ligation 1 and 2. Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.

Following ligations:

  1. LIG1: Tube 4 (insert) + Tube 5
  2. Lig2: Tube 5 (insert) + Tube 9