ULB/7 September 2009
From 2009.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
- | + | Results of ligation: | |
- | + | ||
- | + | * Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick. | |
- | *Colonies | + | |
- | * | + | * Phenotypic tests: |
- | ** | + | |
- | **Comparaison | + | ** Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance. |
- | + | ** Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste. | |
- | Transformation | + | |
- | + | Extracting of plasmides 9, 10, 5, 6 and 2. | |
- | * | + | |
- | + | Transformation of 5 ligations carried out on 9/5. | |
- | Ligation | + | |
- | + | Dubbing of the ligation 1 with IPTG in IPTG medium. | |
- | # | + | |
- | #Lig2 : Tube 5 (insert) + Tube 9 | + | * Results:'''No fluorescence''' <br/> |
- | + | Spreading of a large quantity of ligation 1 and 2. | |
+ | Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator. | ||
+ | |||
+ | Following ligations: | ||
+ | # LIG1: Tube 4 (insert) + Tube 5 | ||
+ | # Lig2: Tube 5 (insert) + Tube 9 |
Revision as of 21:22, 30 September 2009
Results of ligation:
- Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.
- Phenotypic tests:
- Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.
- Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.
Extracting of plasmides 9, 10, 5, 6 and 2.
Transformation of 5 ligations carried out on 9/5.
Dubbing of the ligation 1 with IPTG in IPTG medium.
- Results:No fluorescence
Spreading of a large quantity of ligation 1 and 2. Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.
Following ligations:
- LIG1: Tube 4 (insert) + Tube 5
- Lig2: Tube 5 (insert) + Tube 9