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ULB/9 July 2009
From 2009.igem.org
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html body div.header div.banner { | html body div.header div.banner { | ||
height: 171px; | height: 171px; | ||
| - | background-image: url(https://static.igem.org/mediawiki/2009/ | + | background-image: url(https://static.igem.org/mediawiki/2009/0/0a/Logoulb1.png); |
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
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<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | ||
<li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | ||
| - | <li><a class="mainlink" href="/Team:ULB-Brussels/ | + | <li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li> |
<li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | <li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | ||
<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | ||
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table.calendar { margin: 0; padding: 10px; } | table.calendar { margin: 0; padding: 10px; } | ||
table.calendar td { margin: 0; padding: 2px; vertical-align: top; } | table.calendar td { margin: 0; padding: 2px; vertical-align: top; } | ||
| - | table.month .heading td { padding:2px; background-color:# | + | table.month .heading td { padding:2px; background-color:#3E6DA4; color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } |
table.month .dow td { color:#aaa; text-align:center; font-size:110%; } | table.month .dow td { color:#aaa; text-align:center; font-size:110%; } | ||
table.month td.today { background-color:#ddd; } | table.month td.today { background-color:#ddd; } | ||
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padding: 1pt 1.5pt; | padding: 1pt 1.5pt; | ||
font-weight: bold; | font-weight: bold; | ||
| - | font-size: | + | font-size: 12pt; |
text-align: right; | text-align: right; | ||
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#bodyContent table.month a { background:none; padding:0 } | #bodyContent table.month a { background:none; padding:0 } | ||
.day-active { color:blue } | .day-active { color:blue } | ||
| - | .day-empty { color: | + | .day-empty { color:grey } |
</style> | </style> | ||
</html> | </html> | ||
| + | |||
| + | {| align="right" | ||
| + | {| style="color:#0c6;background-color:#FFFFFF;" cellpadding="3" cellspacing="1" border="1" bordercolor="#035e7b" align="right" | ||
| + | |||
| + | !align="right"|{{#calendar: title=ULB |year=2009 | month=07}} | ||
| + | |- | ||
| + | !align="right"|{{#calendar: title=ULB |year=2009 | month=08}} | ||
| + | |- | ||
| + | !align="right"|{{#calendar: title=ULB |year=2009 | month=09}} | ||
| + | |||
| + | |} | ||
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Choice of bacteria strains : | Choice of bacteria strains : | ||
* ''Caulobacter crescentus'' (strain CB15N) | * ''Caulobacter crescentus'' (strain CB15N) | ||
| - | * ''E. Coli'' (strain K12 or DG1 | + | * ''E. Coli'' (strain K12 or DG1) |
| + | |||
| + | === Constructions === | ||
| + | |||
| + | '''Construction 1''' | ||
| + | |||
| + | As ''Caulobacter crescentus'' genes of interest have'' E. coli'' homologs, we’ll first transfer '''HfsG''' et '''HfsH''' genes through the [http://www.partsregistry.org/Assembly_standard_21 assembly standard 21] and we hope ''E. Coli'' will be able to produce the adhesive material. | ||
| + | |||
| + | Forward and reverse primers are designed. | ||
| + | |||
| + | hfsG and hfsH share a single nucleotide HfsG : ---TG'''A''' ; HfsH : '''A'''TG--- | ||
| + | ==> a blunt-end ligation(?) is possible. | ||
| + | |||
| + | Terminator chosen : [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] | ||
Latest revision as of 21:54, 20 October 2009
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Choice of bacteria strains :
- Caulobacter crescentus (strain CB15N)
- E. Coli (strain K12 or DG1)
Constructions
Construction 1
As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly standard 21 and we hope E. Coli will be able to produce the adhesive material.
Forward and reverse primers are designed.
hfsG and hfsH share a single nucleotide HfsG : ---TGA ; HfsH : ATG--- ==> a blunt-end ligation(?) is possible.
Terminator chosen : BBa_B0015
