ULB/9 July 2009
From 2009.igem.org
(Difference between revisions)
(2 intermediate revisions not shown) | |||
Line 40: | Line 40: | ||
html body div.header div.banner { | html body div.header div.banner { | ||
height: 171px; | height: 171px; | ||
- | background-image: url(https://static.igem.org/mediawiki/2009/ | + | background-image: url(https://static.igem.org/mediawiki/2009/0/0a/Logoulb1.png); |
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
} | } | ||
Line 264: | Line 264: | ||
<li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Project">Project</a></li> | ||
<li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Parts">Parts</a></li> | ||
- | <li><a class="mainlink" href="/Team:ULB-Brussels/ | + | <li><a class="mainlink" href="/Team:ULB-Brussels/Safety">Safety</a></li> |
<li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | <li><a class="mainlink selection" href="/Team:ULB-Brussels/Notebook">Notebook</a></li> | ||
<li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | <li><a class="mainlink" href="/Team:ULB-Brussels/Sponsors">Sponsors</a></li> | ||
Line 309: | Line 309: | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
<html> | <html> | ||
<style type="text/css"> | <style type="text/css"> | ||
Line 372: | Line 346: | ||
|} | |} | ||
+ | |||
Latest revision as of 21:54, 20 October 2009
| ||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ||||||||||||||||||||||||||||||||||||||||||||||||||
|
Choice of bacteria strains :
- Caulobacter crescentus (strain CB15N)
- E. Coli (strain K12 or DG1)
Constructions
Construction 1
As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly standard 21 and we hope E. Coli will be able to produce the adhesive material.
Forward and reverse primers are designed.
hfsG and hfsH share a single nucleotide HfsG : ---TGA ; HfsH : ATG--- ==> a blunt-end ligation(?) is possible.
Terminator chosen : BBa_B0015