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ULB/9 July 2009
From 2009.igem.org
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| + | == July 8-9, 2009 == | ||
Choice of bacteria strains : | Choice of bacteria strains : | ||
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* ''Caulobacter crescentus'' (strain CB15N) | * ''Caulobacter crescentus'' (strain CB15N) | ||
* ''E. Coli'' (strain K12 or DG1 | * ''E. Coli'' (strain K12 or DG1 | ||
| + | |||
| + | === Constructions === | ||
| + | |||
| + | ==== Construction 1 ==== | ||
| + | |||
| + | As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly standart 21 [figure 1] and we hope E. Coli will be able to produce the adhesive material. | ||
| + | |||
| + | Forward and reverse primers are designed. | ||
| + | |||
| + | hfsG and hfsH share a single nucleotide HfsG : ---TG'''A''' ; HfsH : '''A'''TG--- | ||
| + | <br/>=> a blunt-end ligation(?) is possible | ||
| + | |||
| + | Terminator chosen : [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] | ||
Revision as of 08:37, 19 August 2009
July 8-9, 2009
Choice of bacteria strains :
- Caulobacter crescentus (strain CB15N)
- E. Coli (strain K12 or DG1
Constructions
Construction 1
As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly standart 21 [figure 1] and we hope E. Coli will be able to produce the adhesive material.
Forward and reverse primers are designed.
hfsG and hfsH share a single nucleotide HfsG : ---TGA ; HfsH : ATG---
=> a blunt-end ligation(?) is possible
Terminator chosen : BBa_B0015
