"
ULB/9 July 2009
From 2009.igem.org
(Difference between revisions)
| Line 335: | Line 335: | ||
</style> | </style> | ||
</html> | </html> | ||
| - | |||
| - | |||
| - | |||
Choice of bacteria strains : | Choice of bacteria strains : | ||
Revision as of 09:44, 1 October 2009
Choice of bacteria strains :
- Caulobacter crescentus (strain CB15N)
- E. Coli (strain K12 or DG1)
Constructions
Construction 1
As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the [http://www.partsregistry.org/Assembly_standard_21 assembly standard 21] and we hope E. Coli will be able to produce the adhesive material.
Forward and reverse primers are designed.
hfsG and hfsH share a single nucleotide HfsG : ---TGA ; HfsH : ATG--- ==> a blunt-end ligation(?) is possible.
Terminator chosen : [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
