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ULB/9 July 2009
From 2009.igem.org
(Difference between revisions)
| Line 345: | Line 345: | ||
=== Constructions === | === Constructions === | ||
| - | + | '''Construction 1''' | |
| - | As ''Caulobacter crescentus'' genes of interest have'' E. coli'' homologs, we’ll first transfer '''HfsG''' et '''HfsH''' genes through the assembly standard 21 | + | As ''Caulobacter crescentus'' genes of interest have'' E. coli'' homologs, we’ll first transfer '''HfsG''' et '''HfsH''' genes through the [http://www.partsregistry.org/Assembly_standard_21 assembly standard 21] and we hope ''E. Coli'' will be able to produce the adhesive material. |
Forward and reverse primers are designed. | Forward and reverse primers are designed. | ||
hfsG and hfsH share a single nucleotide HfsG : ---TG'''A''' ; HfsH : '''A'''TG--- | hfsG and hfsH share a single nucleotide HfsG : ---TG'''A''' ; HfsH : '''A'''TG--- | ||
| - | + | ==> a blunt-end ligation(?) is possible. | |
Terminator chosen : [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] | Terminator chosen : [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] | ||
Revision as of 09:43, 1 October 2009
Choice of bacteria strains :
- Caulobacter crescentus (strain CB15N)
- E. Coli (strain K12 or DG1)
Constructions
Construction 1
As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the [http://www.partsregistry.org/Assembly_standard_21 assembly standard 21] and we hope E. Coli will be able to produce the adhesive material.
Forward and reverse primers are designed.
hfsG and hfsH share a single nucleotide HfsG : ---TGA ; HfsH : ATG--- ==> a blunt-end ligation(?) is possible.
Terminator chosen : [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015]
