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ULB/9 July 2009
From 2009.igem.org
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Choice of bacteria strains : | Choice of bacteria strains : | ||
* ''Caulobacter crescentus'' (strain CB15N) | * ''Caulobacter crescentus'' (strain CB15N) | ||
| - | * ''E. Coli'' (strain K12 or DG1 | + | * ''E. Coli'' (strain K12 or DG1) |
=== Constructions === | === Constructions === | ||
| - | + | *'''Construction 1''' | |
| - | As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly | + | As ''Caulobacter crescentus'' genes of interest have'' E. coli'' homologs, we’ll first transfer '''HfsG''' et '''HfsH''' genes through the assembly standard 21 [figure 1] and we hope E. Coli will be able to produce the adhesive material. |
Forward and reverse primers are designed. | Forward and reverse primers are designed. | ||
Revision as of 09:38, 1 October 2009
Choice of bacteria strains :
- Caulobacter crescentus (strain CB15N)
- E. Coli (strain K12 or DG1)
Constructions
- Construction 1
As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly standard 21 [figure 1] and we hope E. Coli will be able to produce the adhesive material.
Forward and reverse primers are designed.
hfsG and hfsH share a single nucleotide HfsG : ---TGA ; HfsH : ATG---
=> a blunt-end ligation(?) is possible
Terminator chosen : BBa_B0015
