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ULB/9 July 2009
From 2009.igem.org
(Difference between revisions)
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=== Constructions === | === Constructions === | ||
| - | + | '''Construction 1''' | |
| - | As ''Caulobacter crescentus'' genes of interest have'' E. coli'' homologs, we’ll first transfer '''HfsG''' et '''HfsH''' genes through the assembly standard 21 | + | As ''Caulobacter crescentus'' genes of interest have'' E. coli'' homologs, we’ll first transfer '''HfsG''' et '''HfsH''' genes through the [http://www.partsregistry.org/Assembly_standard_21 assembly standard 21] and we hope ''E. Coli'' will be able to produce the adhesive material. |
Forward and reverse primers are designed. | Forward and reverse primers are designed. | ||
hfsG and hfsH share a single nucleotide HfsG : ---TG'''A''' ; HfsH : '''A'''TG--- | hfsG and hfsH share a single nucleotide HfsG : ---TG'''A''' ; HfsH : '''A'''TG--- | ||
| - | + | ==> a blunt-end ligation(?) is possible. | |
Terminator chosen : [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] | Terminator chosen : [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] | ||
Revision as of 09:43, 1 October 2009
Choice of bacteria strains :
- Caulobacter crescentus (strain CB15N)
- E. Coli (strain K12 or DG1)
Constructions
Construction 1
As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly standard 21 and we hope E. Coli will be able to produce the adhesive material.
Forward and reverse primers are designed.
hfsG and hfsH share a single nucleotide HfsG : ---TGA ; HfsH : ATG--- ==> a blunt-end ligation(?) is possible.
Terminator chosen : BBa_B0015
