ULB/9 July 2009

From 2009.igem.org

(Difference between revisions)
Line 40: Line 40:
html body div.header div.banner {
html body div.header div.banner {
height: 171px;
height: 171px;
-
background-image: url(https://static.igem.org/mediawiki/2009/9/9c/ViewimageuD2vp1.png);
+
background-image: url(https://static.igem.org/mediawiki/2009/0/0a/Logoulb1.png);
     background-repeat: no-repeat;
     background-repeat: no-repeat;
}
}

Revision as of 14:32, 14 October 2009

iGEM Team:ULB-Brussels Wiki


July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30


Choice of bacteria strains :

  • Caulobacter crescentus (strain CB15N)
  • E. Coli (strain K12 or DG1)

Constructions

Construction 1

As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly standard 21 and we hope E. Coli will be able to produce the adhesive material.

Forward and reverse primers are designed.

hfsG and hfsH share a single nucleotide HfsG : ---TGA  ; HfsH : ATG--- ==> a blunt-end ligation(?) is possible.

Terminator chosen : BBa_B0015