ULB/9 September 2009

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(New page: • Extractions des plasmides des 5 ligationsConcentrations obtenues par photométrie au alentour de 20 ng. Nous les avons tout de même utilisés dans la suite des manips, mais ces ext...)
 
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• Extractions des plasmides des 5 ligationsConcentrations obtenues par photométrie au alentour de 20 ng. Nous les avons tout de même utilisés dans la suite des manips, mais ces extractions seront refaites demain. La migration sur gel n’a rien donné.
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• On a fait migrer la digestion des plasmides contenant la construction de la colle Aucune bande n’apparaît à nouveau. :-S
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<title>iGEM Team:ULB-Brussels Wiki</title>
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/*  Design for https://2009.igem.org/Team:ULB-Brussels
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• Les transformations du tube 4 avec le tube 5 ont été repiquées.
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    Université Libre de Bruxelles
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*/
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• Les transformations du tube 10 avec le tube 9 n’ont rien donné. Nous avons donc recommencé cette ligation. Appelée lig B
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html body {
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• Nous avons ligué la ligation 1 avec la ligation 2 (soit les 2 premières constructions du circuit). Appelée lig A.
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html body div.header {
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• Nous avons coulé des boites avec un gradient d’aspartate dans le but de pouvoir observer l’effet de ce chémoatractant sur nos bactéries.
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/* ===== banner ===== */
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• Les nouvelles ligation (transformation) de PRR4 avec GHT ont été repiquées dans 5 tubes, et d’autres colonies de colle ont encore été repiquées.
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• Nous avons fait un test sur les billes collées, soit les immerger dans l’eau. Résultat : ça n’a pas tenu une heure !
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*Plasmid extraction of 5 ligations : concentrations obtained by photometry at around 20 ng.  The gel migration of ligations has failed.
 +
*we have migrated digestion of plasmids containing the construction glue : Results : No band appears again .
 +
*The transformations of the tube 4 to tube 5 were transplanted.
 +
* The transformations of the tube 10 with the tube 9 have given nothing and we started this ligation Called  '''lig B'''
 +
* We have league 1 with the ligation ligation 2 (the first 2 buildings of the circuit). we called '''lig A'''.
 +
* We cast boxes with a gradient of aspartate in order to observe chemotactism of  bacterium.

Latest revision as of 10:25, 21 October 2009

iGEM Team:ULB-Brussels Wiki


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  • Plasmid extraction of 5 ligations : concentrations obtained by photometry at around 20 ng. The gel migration of ligations has failed.
  • we have migrated digestion of plasmids containing the construction glue : Results : No band appears again .
  • The transformations of the tube 4 to tube 5 were transplanted.
  • The transformations of the tube 10 with the tube 9 have given nothing and we started this ligation Called lig B
  • We have league 1 with the ligation ligation 2 (the first 2 buildings of the circuit). we called lig A.
  • We cast boxes with a gradient of aspartate in order to observe chemotactism of bacterium.
Retrieved from "http://2009.igem.org/ULB/9_September_2009"