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ULB/9 September 2009
From 2009.igem.org
(Difference between revisions)
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*we have migrated digestion of plasmids containing the construction glue : Results : No band appears again . | *we have migrated digestion of plasmids containing the construction glue : Results : No band appears again . | ||
*The transformations of the tube 4 to tube 5 were transplanted. | *The transformations of the tube 4 to tube 5 were transplanted. | ||
| - | * The transformations of the tube 10 with the tube 9 have given nothing | + | * The transformations of the tube 10 with the tube 9 have given nothing and we started this ligation Called '''lig B''' |
| - | * We have league 1 with the ligation ligation 2 (the first 2 buildings of the circuit). we called ''lig A''. | + | * We have league 1 with the ligation ligation 2 (the first 2 buildings of the circuit). we called '''lig A'''. |
* We cast boxes with a gradient of aspartate in order to observe chemotactism of bacterium. | * We cast boxes with a gradient of aspartate in order to observe chemotactism of bacterium. | ||
Revision as of 09:17, 7 October 2009
- Plasmid extraction of 5 ligations : concentrations obtained by photometry at around 20 ng. The gel migration of ligations has failed.
- we have migrated digestion of plasmids containing the construction glue : Results : No band appears again .
- The transformations of the tube 4 to tube 5 were transplanted.
- The transformations of the tube 10 with the tube 9 have given nothing and we started this ligation Called lig B
- We have league 1 with the ligation ligation 2 (the first 2 buildings of the circuit). we called lig A.
- We cast boxes with a gradient of aspartate in order to observe chemotactism of bacterium.
