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ULB/9 September 2009
From 2009.igem.org
• Plasmid extraction of 5 ligations : concentrations obtained by photometry at around 20 ng. The gel migration of ligations has failed. • we have migrated digestion of plasmids containing the construction glue : Results : No band appears again . • The transformations of the tube 4 to tube 5 were transplanted(répiquage????). • The transformations of the tube 10 with the tube 9 have given nothing. So we started this ligation. Called B lig • We have league 1 with the ligation ligation 2 (the first 2 buildings of the circuit). we called lig A. • We cast boxes with a gradient of aspartate in order to observe chemotactism of bacterium.
