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Uppsala-Sweden/27 July 2009
From 2009.igem.org
2009-07-27 11:15,OD measurement of Butanol at 750nm,
pirA PCR was conducted, using 1. Primer:pirA forward
pirA reverse
pirB forward
at three different template concentration,
2. Primer:pirA reverse
pirB forward at three different template concentration,
Control pirB
PCR PROGRAM denaturing temperature 98 denaturing time: 30s cycle: 5 denaturing temperature 98 denaturing time: 10s anealing temperature: 63, time 30s extension time:32s extension temperature 72 cycle: 30 denaturing temperature 98 denaturing time: 10s annealing temperature: 72,time 30s extension time:32s extension temperature 72 hold 5 min
-- Emily
