User:DavidC/11 October 2009

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(Difference between revisions)
(→Cell culture)
 
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BBa_B0014; <br>
BBa_B0014; <br>
BBa_ B0030 + BBa_C0012 + BBa_B0014. <br>
BBa_ B0030 + BBa_C0012 + BBa_B0014. <br>
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 +
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==== DNA electrophoresis ====
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==== Test of BBa_B0030 + BBa_C0012 + BBa_B0014 ====
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85 Volt, 15 minutes. <br>
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105 Volt, 40 minutes. <br>
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Ladder fermentas 1 Kb. <br>
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Samples: BBa_B0030 + BBa_C0012 + BBa_B0014.
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<br>
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[[image:SU1110.png|center]]
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<br>

Latest revision as of 17:30, 21 October 2009

framless



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Contents

Sunday the 11th

DNA extraction:

Extraction of p53 from E.coli DH5alpha using a maxiprep (macherey nagel) (2 times).

Cell culture

Growth of E.coli into 5mL of LB medium with 5µL of the correct antibiotic (ampicillin 50mg/mL, kanamycin 50mg/mL or tretracycline 12,5mg/mL).

Samples:
BBa_B0030;
BBa_C0012;
BBa_B0014;
BBa_ B0030 + BBa_C0012 + BBa_B0014.


DNA electrophoresis

Test of BBa_B0030 + BBa_C0012 + BBa_B0014

85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.

Samples: BBa_B0030 + BBa_C0012 + BBa_B0014.

SU1110.png


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