User:DavidC/18 October 2009

From 2009.igem.org

(Difference between revisions)
 
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5mL of LB medium + 5µL of correct antibiotic (kanamycin = 50mg/mL, ampicillin = 50mg/mL and tetracycline = 12,5mg/mL). <br>
5mL of LB medium + 5µL of correct antibiotic (kanamycin = 50mg/mL, ampicillin = 50mg/mL and tetracycline = 12,5mg/mL). <br>
 +
 +
==== DNA extraction ====
 +
 +
By using miniprep from promega:
 +
 +
BBa_J23100 + BBa_B0014; <br>
 +
BBa_B0030 + BBa_J23100 + BBa_B0014; <br>
 +
BBa_R0010 + BBa_B0030; <br>
 +
BBa_P1003 + BBa_B0014;  <br>
 +
BBa_B0014 + BBa_P1003 + BBa_B0014; <br>
 +
BBa_C0012 + BBa_B0014; <br>
 +
BBa_B0030 + BBa_C0012 + BBa_B0014; <br>
 +
BBa_I712669 + BBa_B0014; <br>
 +
D protein from lambda phage
 +
 +
==== Quantification ====
 +
 +
Quantification of miniprep with an electrophoresis. <br>
 +
Mix : 2µL of loading buffer + 2 µL of DNA. <br>
 +
 +
85 Volt, 15 minutes. <br>
 +
105 Volt, 40 minutes. <br>
 +
Ladder fermentas 1 Kb. <br>

Latest revision as of 04:41, 21 October 2009

framless



August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31


Tuesday the 13th to Sunday the 18th

Growth of bacteria in the goal to extract the bio-bricks:

BBa_J23100 + BBa_B0014;
BBa_B0030 + BBa_J23100 + BBa_B0014;
BBa_R0010 + BBa_B0030;
BBa_P1003 + BBa_B0014;
BBa_B0014 + BBa_P1003 + BBa_B0014;
BBa_C0012 + BBa_B0014;
BBa_B0030 + BBa_C0012 + BBa_B0014;
BBa_I712669 + BBa_B0014;

5mL of LB medium + 5µL of correct antibiotic (kanamycin = 50mg/mL, ampicillin = 50mg/mL and tetracycline = 12,5mg/mL).

DNA extraction

By using miniprep from promega:

BBa_J23100 + BBa_B0014;
BBa_B0030 + BBa_J23100 + BBa_B0014;
BBa_R0010 + BBa_B0030;
BBa_P1003 + BBa_B0014;
BBa_B0014 + BBa_P1003 + BBa_B0014;
BBa_C0012 + BBa_B0014;
BBa_B0030 + BBa_C0012 + BBa_B0014;
BBa_I712669 + BBa_B0014;
D protein from lambda phage

Quantification

Quantification of miniprep with an electrophoresis.
Mix : 2µL of loading buffer + 2 µL of DNA.

85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.