User:DavidC/29 September 2009

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framless



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Contents

Tuesday the 29th

Ligation between D protein and adenovirus 5 penton base

Restriction digest of D protein by Spe I (380bp):
DNA (PCR with bio-brick primers) = 5µL
Buffer M (TAKARA) =2µL
BSA (TAKARA) = 2µL <br H20 = 10µL
Spe 1 (TAKARA) = 1µL
1 hour of incubation at 37°C.

Restriction digest of adenovirus 5 penton base by XbaI (1715bp):

DNA (PCR with bio-brick primers) = 5µL
Buffer M (TAKARA) =2µL
BSA (TAKARA) = 2µL <br H20 = 10µL
Xba 1 (TAKARA) = 1µL
1 hour of incubation at 37°C.

DNA electrophoresis

85 Volt, 15 minutes.
105 Volt, 40 minutes.
Ladder fermentas 1 Kb.


Samples:

TU2909.png


DNA purification

Kit Qiagen “gel extraction kit”, final volume = 50µL.