Week1

From 2009.igem.org

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1.LB and M9 Preparation
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1.LB and M9 Medium  Preparation  
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2.Ampicillin stock-100X (X=100 microg/ml)
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2.Ampicillin stock-100X (X=100 microg/ml)  
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3.Growth Curve of CFP and YFP  
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3.Growth Curve of strains having CFP and YFP on LB and M( medium.
4.Trial FACS run with 100microM of IPTG for CFP and YFP (without controls)- signal obtained
4.Trial FACS run with 100microM of IPTG for CFP and YFP (without controls)- signal obtained
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*henceforth all the FACS runs were with proper controls
 

Latest revision as of 01:36, 22 October 2009

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1.LB and M9 Medium Preparation

2.Ampicillin stock-100X (X=100 microg/ml)

3.Growth Curve of strains having CFP and YFP on LB and M( medium.

4.Trial FACS run with 100microM of IPTG for CFP and YFP (without controls)- signal obtained

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