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Week3
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
1.M9 media preparation | 1.M9 media preparation | ||
| - | 2.IPTG stock | + | 2.1M IPTG stock |
3.First FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: blank contaminated) | 3.First FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: blank contaminated) | ||
4.Second FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: sample stored after pelleting) | 4.Second FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: sample stored after pelleting) | ||
Revision as of 09:33, 22 July 2009
1.M9 media preparation
2.1M IPTG stock
3.First FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: blank contaminated)
4.Second FACS run with 100 microM IPTG for CFP and YFP- no signal obtained (reason: sample stored after pelleting)
