Week5
From 2009.igem.org
1.FACS run with different conc. of IPTG (100,250,500 microM/ml) for CFP and YFP-no signal(reason: sample stored after pelleting)
2.FACS run with different conc. of IPTG (100,250,500 microM/ml) for CFP and YFP-
signal obtained for YFP with gradation as per IPTG conc.
no signal obtained for CFP
- CFP culture problem suspected