Wisconsin-Madison/25 August 2009

From 2009.igem.org

(Difference between revisions)
(New page: == '''Calendar''' == <table> <html> <style type="text/css"> table.calendar { margin: 0; padding: 4px; } table.calendar td { margin: 0; padding: 4px; vertical-align: top; } ...)
 
Line 61: Line 61:
Confirm sequencing results that Gssdmt worked.
Confirm sequencing results that Gssdmt worked.
 +
 +
'''Cyanobacteria''
 +
* optimum OD 685 - PCC 7002
 +
* reinoculated all samples in 5:1 ratios

Latest revision as of 19:53, 20 October 2009

Calendar

April
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

August 25, 2009

Sequencing Reaction on Gssdmt

.5 uL DNA (100 ng)

1 uL Big Dye

1.5uL Big Dye Buffer

.25uL Primer (fwd or rev)

5.75uL H2O


Sequencing clean-up using magnetic beads


Confirm sequencing results that Gssdmt worked.

'Cyanobacteria

  • optimum OD 685 - PCC 7002
  • reinoculated all samples in 5:1 ratios