Wisconsin-Madison/25 August 2009
From 2009.igem.org
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+ | * optimum OD 685 - PCC 7002 | ||
+ | * reinoculated all samples in 5:1 ratios |
Latest revision as of 19:53, 20 October 2009
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August 25, 2009
Sequencing Reaction on Gssdmt
.5 uL DNA (100 ng)
1 uL Big Dye
1.5uL Big Dye Buffer
.25uL Primer (fwd or rev)
5.75uL H2O
Sequencing clean-up using magnetic beads
Confirm sequencing results that Gssdmt worked.
'Cyanobacteria
- optimum OD 685 - PCC 7002
- reinoculated all samples in 5:1 ratios