Illinois/21 July 2009

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June
MTWTFSS
[http://2009.igem.org/Illinois/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Illinois/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Illinois/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Illinois/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_June_2009&action=edit 14]
[http://2009.igem.org/Illinois/15_June_2009 15] [http://2009.igem.org/Illinois/16_June_2009 16] [http://2009.igem.org/Illinois/17_June_2009 17] [http://2009.igem.org/Illinois/18_June_2009 18] [http://2009.igem.org/Illinois/19_June_2009 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Illinois/22_June_2009&action=edit 22] [http://2009.igem.org/Illinois/23_June_2009 23] [http://2009.igem.org/Illinois/24_June_2009 24] [http://2009.igem.org/Illinois/25_June_2009 25] [http://2009.igem.org/Illinois/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_June_2009&action=edit 28]
[http://2009.igem.org/Illinois/29_June_2009 29] [http://2009.igem.org/Illinois/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Illinois/1_July_2009 1] [http://2009.igem.org/Illinois/2_July_2009 2] [http://2009.igem.org/Illinois/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_July_2009&action=edit 5]
[http://2009.igem.org/Illinois/6_July_2009 6] [http://2009.igem.org/Illinois/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_July_2009&action=edit 8] [http://2009.igem.org/Illinois/9_July_2009 9] [http://2009.igem.org/Illinois/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_July_2009&action=edit 12]
[http://2009.igem.org/Illinois/13_July_2009 13] [http://2009.igem.org/Illinois/14_July_2009 14] [http://2009.igem.org/Illinois/15_July_2009 15] [http://2009.igem.org/Illinois/16_July_2009 16] [http://2009.igem.org/Illinois/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_July_2009&action=edit 19]
[http://2009.igem.org/Illinois/20_July_2009 20] [http://2009.igem.org/Illinois/21_July_2009 21] [http://2009.igem.org/Illinois/22_July_2009 22] [http://2009.igem.org/Illinois/23_July_2009 23] [http://2009.igem.org/Illinois/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Illinois/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Illinois/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Illinois/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_August_2009&action=edit 2]
[http://2009.igem.org/wiki/index.php?title=Illinois/3_August_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_August_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_August_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_August_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_August_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=Illinois/10_August_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_August_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_August_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_August_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_August_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Illinois/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Illinois/16_August_2009&action=edit 16]
[http://2009.igem.org/wiki/index.php?title=Illinois/17_August_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_August_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_August_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Illinois/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Illinois/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=Illinois/24_August_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_August_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_August_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_August_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_August_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=Illinois/31_August_2009&action=edit 31]

July 21

Hybrid Promoter

Finished overnight digestion and digested with SpeI

Modeling

We may have determined why our plasmid backbone pSB3K3 has been turning up in gels as 2kbp rather than 2.75kbp. There is a [http://partsregistry.org/Help:Plasmid_Backbone_Availability_2009 Parts Registry link] that seems to indicate that the pSB3K3 backbone with the two Biobricks we have been using has problems. It also suggested an alternative Biobrick in the kit. We ran PCRs on the old Biobrick with this backbone, the Biobrick suggested by the Parts Registry, and a third Biobrick with the backbone from the 2008 Parts Distribution. Unfortunately, our gel indicated that the PCRs were unsuccessful. We will have to try again tomorrow.

PSB3K3 PCR 7-21.jpg


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