Illinois/22 July 2009

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June
MTWTFSS
[http://2009.igem.org/Illinois/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Illinois/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Illinois/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Illinois/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_June_2009&action=edit 14]
[http://2009.igem.org/Illinois/15_June_2009 15] [http://2009.igem.org/Illinois/16_June_2009 16] [http://2009.igem.org/Illinois/17_June_2009 17] [http://2009.igem.org/Illinois/18_June_2009 18] [http://2009.igem.org/Illinois/19_June_2009 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Illinois/22_June_2009&action=edit 22] [http://2009.igem.org/Illinois/23_June_2009 23] [http://2009.igem.org/Illinois/24_June_2009 24] [http://2009.igem.org/Illinois/25_June_2009 25] [http://2009.igem.org/Illinois/26_June_2009 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_June_2009&action=edit 28]
[http://2009.igem.org/Illinois/29_June_2009 29] [http://2009.igem.org/Illinois/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Illinois/1_July_2009 1] [http://2009.igem.org/Illinois/2_July_2009 2] [http://2009.igem.org/Illinois/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_July_2009&action=edit 5]
[http://2009.igem.org/Illinois/6_July_2009 6] [http://2009.igem.org/Illinois/7_July_2009 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_July_2009&action=edit 8] [http://2009.igem.org/Illinois/9_July_2009 9] [http://2009.igem.org/Illinois/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_July_2009&action=edit 12]
[http://2009.igem.org/Illinois/13_July_2009 13] [http://2009.igem.org/Illinois/14_July_2009 14] [http://2009.igem.org/Illinois/15_July_2009 15] [http://2009.igem.org/Illinois/16_July_2009 16] [http://2009.igem.org/Illinois/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_July_2009&action=edit 19]
[http://2009.igem.org/Illinois/20_July_2009 20] [http://2009.igem.org/Illinois/21_July_2009 21] [http://2009.igem.org/Illinois/22_July_2009 22] [http://2009.igem.org/Illinois/23_July_2009 23] [http://2009.igem.org/Illinois/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_July_2009&action=edit 26]
[http://2009.igem.org/wiki/index.php?title=Illinois/27_July_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Illinois/31_July_2009&action=edit 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Illinois/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Illinois/2_August_2009&action=edit 2]
[http://2009.igem.org/wiki/index.php?title=Illinois/3_August_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Illinois/4_August_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Illinois/5_August_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Illinois/6_August_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Illinois/7_August_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Illinois/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Illinois/9_August_2009&action=edit 9]
[http://2009.igem.org/wiki/index.php?title=Illinois/10_August_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Illinois/11_August_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Illinois/12_August_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Illinois/13_August_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Illinois/14_August_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Illinois/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Illinois/16_August_2009&action=edit 16]
[http://2009.igem.org/wiki/index.php?title=Illinois/17_August_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Illinois/18_August_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Illinois/19_August_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Illinois/20_August_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Illinois/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Illinois/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Illinois/23_August_2009&action=edit 23]
[http://2009.igem.org/wiki/index.php?title=Illinois/24_August_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Illinois/25_August_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Illinois/26_August_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Illinois/27_August_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Illinois/28_August_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Illinois/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Illinois/30_August_2009&action=edit 30]
[http://2009.igem.org/wiki/index.php?title=Illinois/31_August_2009&action=edit 31]

July 22

Hybrid Promoter

The gel that was run to inactivate the digestion looked awful.

UI09 DR promoter digestion 7-22.jpg

Reexamined protocol from the fermentas website and decided to attempt a double digest using Spe1 first and then EcoRI

Modeling

We retried PCRs for the pSB3K3 plasmid backbone, using two samples of template: Spring 2009 Plate 2 Well 15L and Spring 2008 Plate 1014 Well 1F. We ran four different PCRs, two for each under two different PCR programs: the program for amplifying PJU-334 and the program suggested for amplifying pSB3K3. Our gel indicated that none of the four PCRs worked.


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