September/17 September 2009

From 2009.igem.org

1.colony check

sample   no. of colony
  45        0
  46        0
  49        0

2.mini prep
result of nano drop

 sample       conc.
  17          14.1 ng/uL
  20-A        22.8
  20-B        24.3
  27          16.7
  28          23.3
  30          26.6
  35          19.4
  37          19.2
  48-A        31.5
  48-B        20.4

3.digestion

K204050

Vector		Insert
1-12A	8	no.17	12
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	8	dH₂O	4
total	20uL	total	20uL

↓
37 degree 3hr
gel electrophoresis
gelcut

4.transformation

sample No. 29 , 38

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