Team:Chiba/Project/reporter

From 2009.igem.org

Time delay depending on the reporters

In our expetriments, the more better condition of medium for the animation demonstration was exxamined with 5 different reporters.

Reporter GFPuv mRFP sfGFP mCherry mOrange
Time of Recognizing the fastest LuxR [h] 2 3.5 4 5 11
Time of Recognizing the last LuxR [h] 3 24.5 50 25.5 71
  • Condition

Medium :agar 5%, Thickness 7.0 mm

Temperature : r.t. (ca. 25 degree)



  • The plate shown in each photos were obtained at 0, 2, 3.5, 4, 5 h and several days after the incubation (from upper left to bottom right).