Team:DTU Denmark/test table

Required function

Constitutive promoter

Constitutive expression of REX-Activator fusion protein

Kozak sequence

Ribosome start-codon recognition and enhanced initiating of translation

REX

REX (redox regulator) that binds to ROP at high NAD+/NADH ratio

Linker

To couple two protein domains without disrupting their individual functions

Activator domain

Protein domain able to activate transcription in eukaryotes in proximity of a minimal promoter.

Nuclear Localization Sequence (NLS)

Translocation of the REX-Activator protein to the nucleus

Terminator 1

Sequence that will terminate transcription

ROB

DNA sequence that REX binds at high NAD+/NADH ratio

Minimal promoter

Promoter devoid of regulatory motifs. Only expression if an activator is bound upstream.

GFP

The amount of green fluprescent protein can be quantitatively measured.

Degradation signal

Ensures fast degradation of GFP

Terminator 2

Terminates transcription. Different from terminator 1 to avoid direct repeats, which can cause problems with PCR-amplifications