Team:Kyoto/CiC/Notebook/0928-1002

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0928-1004 : constructing sig-GFP generator and sig-EGFP generator(HeLa)


Monday, 28 September

To Do

  • sig-EGFP generator(HeLa)
    • Restriction Enzyme Digestion(Hind3)

Results

They were unexpectedly digested by Hind3,the Restriction Enzyme which would not work if the signal sequence were inserted in the vector correctly.



Tuesday, 29 September

To Do

  • sig-GFP generator
    • PCR(the second PCR)
  • sig-EGFP generator(HeLa)
    • phosphorylation signal sequence primer
    • annealing signal sequence primer
    • Restriction Enzyme Digestion(EcoR1 + Xho1)
    • ligation
    • transformation

Results

No colony was observed (090930)



Wednesday, 30 September

To Do

  • sig-GFP generator
    • miniprep I712074(T7 promoter)
    • Restriction Enzyme Digestion
    • ligation
    • transformation
  • sig-EGFP generator(HeLa)
    • ligation
    • transformation

Results

Transformation

Too many colonies were observed that we could find no fignificant differences between the treated cluture the control. (091001)



Thursday, 01 October

To Do

No experiments were undertaken



Friday, 02 October

To Do

No experiments were undertaken



Saturday, 03 October

To Do

No experiments were undertaken



Saturday, 04 October

To Do

No experiments were undertaken



Sunday, 05 October

To Do

No experiments were undertaken