Team:Kyoto/CiC/Notebook/1005-1009

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

1005-1009 : constructing sig-GFP generator,sig-EGFP generator(HeLa)


Monday, 05 October

To Do

No experiments were undertaken



Tuesday, 06 October

To Do

No experiments were undertaken



Wednesday, 07 October

To Do

  • sig-GFP generator
    • Restriction Enzyme
    • ligation
    • transformation
  • sig-EGFP generator
    • phosphorylation signal sequence primer
    • anneling signal sequence primer
    • ligation
    • transformation

Results

Transformation

Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)



Thursday, 08 October

To Do

  • sig-GFP generator
    • Restriction Enzyme
    • ligation
    • transformation
  • sig-EGFP generator
    • phosphorylation signal sequence primer
    • anneling signal sequence primer
    • ligation
    • transformation

Results

Transformation

Sufficient,but not excessive number of colonies were observed,so we decided to move on to next step.(091010)



Friday, 09 October

To Do

  • No experiments were undertaken



Saturday, 10 October

To Do

  • sig-GFP
    • miniprep
    • Restriction Enzyme
  • sig-EGFP(HeLa)
    • miniprep
    • Restriction Enzyme

Results

Plasmid construction is completed!!



Saturday, 11 October

To Do

  • Yeast Mitochondria isolation

Results

  • It is too difficult for us to isolate mitochondoria.



Sunday, 12 October

To Do

  • No experiments were undertaken