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Team:Kyoto/CiC/Notebook/1012-1016
From 2009.igem.org
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
1012-1018 : Obserbation
Monday, 12 October
To Do
No experiments were undertaken
Results
Tuesday, 13 October
To Do
- sequence
- sig-GFP generator
- sig-EGFP generator(HeLa)
Results
- We assured that the plasmid include the correct sequence by analyzing it with sequencer.(091015)
Wednesday, 14 October
To Do
No experiments were undertaken
Thursday, 15 October
To Do
- Experiments to approach subgoalA
- Making of liposome by natural swelling method and extruder
Friday, 16 October
To Do
- Experiments to approach subgoalA
- Making of liposome by natural swelling method and extruder
- expression in vitro HIV-TAT-(LALAAAA)3-generator
- add liposome to HeLa cells
Results
No NBD-PE signal was observed in HeLa cells. This might be because we did not collect the right fraction after density gradient centrifugation and thus lost the liposomes.
Saturday, 17 October
To Do
- Experiments to approach subgoalA
- add liposome including synthesized HIV-TAT-(LALAAAA)3 peptides to HeLa cells
Results
No NBD-PE signal was observed in HeLa cells.
Sunday, 18 October
To Do
- observation by microscope
Results
We could't confirm that liposome was not taken by HeLa cells.
